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hspb5  (Addgene inc)


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    Structured Review

    Addgene inc hspb5
    Hspb5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hspb5/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    hspb5 - by Bioz Stars, 2026-05
    91/100 stars

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    Transcript levels were measured for hspb2, hspb1, hspb5, hspb6 in HSPB2wt and HSPB2cKO animals, after sham or TAC-surgery. The transcript abundance increased significantly in response to pressure-overload. Except for hspb2, there were no significant differences between sHsp mRNA expression in HSPB2wt and HSPB2cKO hearts in both of sham and TAC operated mice. From 3 to 5 heart samples were analyzed per group. *P<0.05compared with each sham groups.

    Journal: PLoS ONE

    Article Title: HSPB2 Is Dispensable for the Cardiac Hypertrophic Response but Reduces Mitochondrial Energetics following Pressure Overload In Mice

    doi: 10.1371/journal.pone.0042118

    Figure Lengend Snippet: Transcript levels were measured for hspb2, hspb1, hspb5, hspb6 in HSPB2wt and HSPB2cKO animals, after sham or TAC-surgery. The transcript abundance increased significantly in response to pressure-overload. Except for hspb2, there were no significant differences between sHsp mRNA expression in HSPB2wt and HSPB2cKO hearts in both of sham and TAC operated mice. From 3 to 5 heart samples were analyzed per group. *P<0.05compared with each sham groups.

    Article Snippet: The proteins were transferred to nitrocellulose membrane and were probed with antibodies against HSPB2 (see description above), CRYAB/HSPB5 antibody (1∶5000) , HSP20 (HSPB6 (ADI-SPA-796, Enzo Life Sciences; 1∶2000), HSP25/HSPB1 (ADi-SPA- 801, Enzo Life Sciences; 1∶1000), VDAC (porin, PC548, Calbiochem: 1∶1000) and GAPDH (GAPDH -14C10, Cell signaling technology; 1∶5000).

    Techniques: Expressing

    Hearts from HSPB2wt and HSPB2cKO were isolated at 8 weeks after sham or TAC procedure and protein extracts (soluble fraction) were analyzed by western blots probed with anti-HSPB2, HSPB1 (HSP25), HSPB5 (CRYAB) and HSPB6 (HSP20). In contrast to transcripts, protein levels were not significantly modified in samples from TAC operated mice and except for HSPB2, there was no difference between HSPB2cKO and HSPB2wt samples. Two to five samples were analyzed per group and a representative example is shown.

    Journal: PLoS ONE

    Article Title: HSPB2 Is Dispensable for the Cardiac Hypertrophic Response but Reduces Mitochondrial Energetics following Pressure Overload In Mice

    doi: 10.1371/journal.pone.0042118

    Figure Lengend Snippet: Hearts from HSPB2wt and HSPB2cKO were isolated at 8 weeks after sham or TAC procedure and protein extracts (soluble fraction) were analyzed by western blots probed with anti-HSPB2, HSPB1 (HSP25), HSPB5 (CRYAB) and HSPB6 (HSP20). In contrast to transcripts, protein levels were not significantly modified in samples from TAC operated mice and except for HSPB2, there was no difference between HSPB2cKO and HSPB2wt samples. Two to five samples were analyzed per group and a representative example is shown.

    Article Snippet: The proteins were transferred to nitrocellulose membrane and were probed with antibodies against HSPB2 (see description above), CRYAB/HSPB5 antibody (1∶5000) , HSP20 (HSPB6 (ADI-SPA-796, Enzo Life Sciences; 1∶2000), HSP25/HSPB1 (ADi-SPA- 801, Enzo Life Sciences; 1∶1000), VDAC (porin, PC548, Calbiochem: 1∶1000) and GAPDH (GAPDH -14C10, Cell signaling technology; 1∶5000).

    Techniques: Isolation, Western Blot, Modification